In general, broad moderate-weak intensity immunostaining of dense axonal network throughtout the brain, but strong staining of axons is noticed in the caudate putamen, nucleus accumbens (core and shell), ventral pallidum, olfactory tubercule, lateral septum, amygdala (especially in the basoilateral part), locus coeruleus-parabrachial region around the fourth ventricle.
Strong intensity cytoplasmic somato-dendritic immunostaining is noticed in a subset of neurons in the medial septum, HDB-VDB, ventral pallidum, substantia innominata, substantia nigra - ventral tegmental area and multiple (but distinct) nuclei of the pons and medulla, like dorsal raphe, oculomotor nucleus, lateral paragigantocellular nucleus, n. ambinguus, n. facialis, motor trigeminus nucleus.
Median eminence is strognly immunreactive.
Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.