ARHGAP26

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035106

 

Antibody HPA035107

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035106 HPA035107 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

78 90 

Antigen sequence

RSSLHAVFSLLVNFVPCHPNLHLLFDRPEEAVHEDSSTPFRKAKALYACK
AEHDSELSFTAGTVFDNVHPSQEPGWLE
 
HLSRKKSSDSKPPSCSERPLTLFHTVQSTEKQEQRNSIINSSLESVSSNP
NSILNSSSSLQPNMNSSDPDLAVVKPTRPNSLPPNPSPTS
 

Matching transcripts

ARHGAP26-001 - ENSP00000274498 [100%]
ARHGAP26-012 - ENSP00000389137 [86%]
 ARHGAP26-001 - ENSP00000274498 [100%]
ARHGAP26-008 - ENSP00000367243 [100%]
ARHGAP26-021 - ENSP00000393276 [100%]
ARHGAP26-019 - ENSP00000413283 [92%]
ARHGAP26-020 - ENSP00000416889 [92%]
ARHGAP26-018 - ENSP00000400565 [91%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
More information
  

Antibody dilution

1:179  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

92.2, 16.2 92.2, 86.2, 42.9, 32.8, 14.0, 13.9 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:30000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in myocytes.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:50 

Validation IH

 Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 
 

ANTIGEN VIEW

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ARHGAP26-001
 
ARHGAP26-008
 
ARHGAP26-012
 
ARHGAP26-018
 
ARHGAP26-019
 
ARHGAP26-020
 
ARHGAP26-021