Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to the nucleus but also to the Golgi apparatus and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA003980, CAB007772, CAB027385
 

Antibody HPA003980

 

Antibody CAB007772

 

Antibody CAB027385

 

         Cell line A-431

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Location(s)

Golgi apparatus  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Speckle 

Staining intensity

Moderate Negative Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm, Golgi apparatus  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Speckle 

Staining intensity

Strong Negative Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty  Speckle 

Staining intensity

Moderate Negative Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of the Golgi apparatus in all three cell lines. Additional staining of cytoplasm and/or nucleus in U-251 MG and U-2 OS.   No staining was observed in any of the three cell lines.   Staining of cytoplasm and nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus