Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Plasma membrane 

Staining summary

Mainly localized to the cytoplasm but also to the plasma membrane.

Reliability (APE)

High

Antibodies in assay

CAB003844, HPA006563, HPA006564, CAB016290
 

Antibody CAB003844

 

Antibody HPA006563

 

Antibody HPA006564

 

Antibody CAB016290

 

         Cell line A-431

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Location(s)

 Cytoplasm Cytoplasm   

Staining characteristics

 Granular Granular   

Staining intensity

Negative Weak Weak   

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   

Comment

     

         Cell line U-2 OS

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Location(s)

 Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm  

Staining characteristics

    

Staining intensity

Negative Strong Strong Negative 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Cytoplasm Cytoplasm  

Staining characteristics

 Granular Granular  

Staining intensity

Negative Weak Weak Negative 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of plasma membrane in U-2 OS.   Staining of cytoplasm in all three cell lines with additional staining of plasma membrane in U-2 OS.   No staining was observed in three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane