ABCA9

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA054823

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA054823 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

29 

Antigen sequence

FWFQHGRANHVVLENETDSDPTPNDCFEP
 

Matching transcripts

ABCA9-001 - ENSP00000342216 [100%]
ABCA9-002 - ENSP00000394264 [100%]
ABCA9-201 - ENSP00000359767 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human heart muscle shows strong nuclear positivity in myocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:3500 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

184.4, 180.0, 162.5 

Antibody dilution

1:940 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:7500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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ABCA9-001
 
ABCA9-002
 
ABCA9-201