Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Plasma membrane, Cytoplasm, Focal Adhesions

Additional location(s)

 

Staining summary

Localized to focal adhesion sites, the cytoplasm and plasma membrane.

Reliability (APE)

High

Antibodies in assay

HPA028448, HPA028696
 

Antibody HPA028448

 

Antibody HPA028696

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm, Focal Adhesions, Cell Junctions Plasma membrane, Cytoplasm, Focal Adhesions 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm, Focal Adhesions Plasma membrane, Cytoplasm, Focal Adhesions 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Plasma membrane, Cytoplasm, Focal Adhesions Plasma membrane, Cytoplasm, Focal Adhesions 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & focal adhesions.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & focal adhesions.
 

Summary

Staining of cytoplasm, plasma membrane and focal adhesions in all three cell lines with additional staining of cell junctions in A-431 and cytoskeleton (actin filaments) in U-2 OS.   Staining of focal adhesion sites, the plasma membrane and cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Focal adhesions
Plasma membrane