Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli 

Staining summary

Mainly localized to cytoplasm but also to nucleoli.

Reliability (APE)

Low

Antibodies in assay

HPA015323, HPA015810
 

Antibody HPA015323

 

Antibody HPA015810

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm  

Staining characteristics

Cluster  

Staining intensity

Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleoli in U-2 OS.   Staining of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleoli