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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Vesicles 

Staining summary

Staining of nucleus and vesicles in all three cell lines with additional staining of plasma membrane in A-431.

Reliability (Single)

Antibodies in assay

HPA038624
 

Antibody HPA038624

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

Speckle 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

Speckle 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & vesicles.
 

Summary

Staining of nucleus and vesicles in all three cell lines with additional staining of plasma membrane in A-431.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli
Plasma membrane
Endosome