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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and nucleus but excluded from the nucleoli, or localized to the nucleoli.

Reliability (APE)

Very low

Antibodies in assay

HPA011913, CAB026456
 

Antibody HPA011913

 

Antibody CAB026456

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleoli 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Nucleoli 

Staining characteristics

   

Staining intensity

  Weak 

Validation

  
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm   

Staining characteristics

Granular   

Staining intensity

Weak   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli.
 

Summary

Staining of nucleus and cytoplasm in U-251 MG and A-431.   Staining of nucleoli in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleoli
Nucleus but not nucleoli