Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Vesicles, Centrosome 

Staining summary

Mainly localized to cytoplasm but also centrosome and vesicles.

Reliability (APE)

High

Antibodies in assay

HPA000247, HPA000834
 

Antibody HPA000247

 

Antibody HPA000834

 

         Cell line A-431

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Location(s)

Cytoplasm, Vesicles Cytoplasm, Centrosome 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Vesicles Cytoplasm 

Staining characteristics

Smooth Granular 

Staining intensity

Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of vesicles in U-2 OS and A-431.   Staining of cytoplasm in all three cell lines. Additional staining of centrosome in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm
Endosome