PEX2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA011410

 

Antibody HPA027729

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA011410 HPA027729 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

131 134 

Antigen sequence

NAKSANRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEV
KACLWVFLWRFTIYSKNATVGQSVLNIKYKNDFSPNLRYQPPSKNQKIWY
AVCTIGGRWLEERCYDLFRNHHLASFGKVKQ
 
LGIHSVFCKPQNIREVGFEYMNRELLWHGFAEFLIFLLPLINVQKLKAKL
SSWCIPLTGAPNSDNTLATSGKECALCGEWPTMPHTIGCEHIFCYFCAKS
SFLFDVYFTCPKCGTEVHSLQPLKSGIEMSEVNA
 

Matching transcripts

PEX2-001 - ENSP00000349543 [100%]
PEX2-002 - ENSP00000428638 [100%]
PEX2-003 - ENSP00000428590 [100%]
PEX2-006 - ENSP00000429304 [100%]
PEX2-201 - ENSP00000400984 [100%]
 PEX2-001 - ENSP00000349543 [99%]
PEX2-002 - ENSP00000428638 [99%]
PEX2-003 - ENSP00000428590 [99%]
PEX2-201 - ENSP00000400984 [99%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
More information
 Immunohistochemical staining of human gall bladder shows distinct cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:10 1:7 

Validation IH

Supportive: Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data Supportive: Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

34.8, 20.8 34.8 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PEX2-001
 
PEX2-002
 
PEX2-003
 
PEX2-006
 
PEX2-201