MELK

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA017214

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA017214 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

142 

Antigen sequence

DIKSNNWSLEDVTASDKNYVAGLIDYDWCEDDLSTGAATPRTSQFTKYWT
ESNGVESKSLTPALCRTPANKLKNKENVYTPKSAVKNEEYFMFPEPKTPV
NKNQHKREILTTPNRYTTPSKARNQCLKETPIKIPVNSTGTD
 

Matching transcripts

MELK-001 - ENSP00000298048 [100%]
MELK-201 - ENSP00000443550 [100%]
MELK-202 - ENSP00000439792 [100%]
MELK-203 - ENSP00000439184 [100%]
MELK-204 - ENSP00000438226 [100%]
MELK-206 - ENSP00000441596 [100%]
MELK-207 - ENSP00000445452 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:1250 

Validation IH

Uncertain: One antibody yielding a staining pattern which is partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402376)
More information
 

Target mass (kDa)

74.6, 71.2, 69.1, 66.5, 66.4, 59.6, 52.5 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:140000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
MELK-001
 
MELK-201
 
MELK-202
 
MELK-203
 
MELK-204
 
MELK-206
 
MELK-207