KRT8

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA049866

 

Antibody CAB000131

 

Antibody CAB001696

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 DakoCytomation
 Chemicon
 

Product name

HPA049866 N1560 MAB3414 

Host species

Rabbit Mouse Mouse 

Clonality

pAb mAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Supernatant Protein A/G 

Released in version

10 1 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known Native protein 

Length (aa)

33   

Antigen sequence

SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
 
 
 

Matching transcripts

KRT8-001 - ENSP00000447566 [100%]
KRT8-002 - ENSP00000293308 [100%]
KRT8-003 - ENSP00000447402 [100%]
KRT8-004 - ENSP00000449404 [100%]
   

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 Immunohistochemical staining of human prostate shows moderate cellmembranous positivity in glandular cells.
More information
 Immunohistochemical staining of human prostate shows strong cellmembranous positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:1000 1:50 1:500 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data 

         Immunofluorescence

Image

   

Description

 Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments).
More information
  

Antibody dilution

 1:13  

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

56.6, 53.7 56.6, 53.7, 32.5, 30.9, 19.6, 19.1, 13.6, 8.9 56.6, 53.7, 32.5, 30.9, 19.6, 19.1, 13.6, 8.9 

Antibody dilution

1:100 1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
   

Antibody dilution

1:850   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

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KRT8-001
 
KRT8-002
 
KRT8-003
 
KRT8-004