Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to intermediate filaments.

Reliability (APE)

High

Antibodies in assay

CAB000131, HPA049866
 

Antibody CAB000131

 

Antibody HPA049866

 

         Cell line A-431

Toggle channels

   

Location(s)

Cytoskeleton (Intermediate filaments)   

Staining characteristics

   

Staining intensity

Weak   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   

Comment

   

         Cell line CACO-2

Toggle channels

  
 

Location(s)

  Cytoskeleton (Intermediate filaments) 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

   

         Cell line MCF-7

Toggle channels

  
 

Location(s)

  Cytoskeleton (Intermediate filaments) 

Staining characteristics

   

Staining intensity

  Strong 

Validation

  
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

   

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Cytoskeleton (Intermediate filaments) Cytoskeleton (Intermediate filaments) 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

  

         Cell line U-251 MG

Toggle channels

   

Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line MCF-7 shows positivity in cytoskeleton (intermediate filaments).
 

Summary

Staining of cytoskeleton (intermediate filaments) in A-431 and U-2 OS.   Staining of cytoskeleton (intermediate filaments) in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Intermediate filaments