Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the cytoplasm but also to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

CAB002145, HPA002655, HPA005990
 

Antibody CAB002145

 

Antibody HPA002655

 

Antibody HPA005990

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Granular Granular Granular 

Staining intensity

Weak Weak Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Cytoplasm Cytoplasm 

Staining characteristics

 Granular Granular 

Staining intensity

Negative Weak Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Negative Weak Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in A-431.   Granular staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli