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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to cytoplasm and intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

HPA023559, HPA023564, HPA044971
 

Antibody HPA023559

 

Antibody HPA023564

 

Antibody HPA044971

 

         Cell line A-431

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Location(s)

    

Staining characteristics

    

Staining intensity

Negative Negative   

Validation

No staining.
 
No staining.
   

Comment

    

         Cell line U-2 OS

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Location(s)

  Cytoplasm Cytoskeleton (Intermediate filaments) 

Staining characteristics

  Granular  

Staining intensity

  Weak Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

    

         Cell line U-251 MG

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Location(s)

  Cytoskeleton (Intermediate filaments) 

Staining characteristics

   

Staining intensity

Negative Negative Strong 

Validation

No staining.
 
No staining.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoskeleton (intermediate filaments).
 

Summary

No staining was observed in any of the three cell lines.   Staining of the cytoplasm in U-2 OS.   Staining of cytoskeleton (intermediate filaments) in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments