Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Focal Adhesions 

Staining summary

Mainly localized to cytoplasm but also focal adhesions.

Reliability (APE)

Medium

Antibodies in assay

HPA003817, HPA015007
 

Antibody HPA003817

 

Antibody HPA015007

 

         Cell line A-431

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Location(s)

Cytoplasm  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Focal Adhesions Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in U-251MG and A-431, with additional staining of focal adhesions in U-251 MG.   Staining of cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Focal adhesions