Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments) 

Staining summary

Mainly localized to the cytoplasm. Additionally localized to the nucleus and intermediate filaments.

Reliability (APE)

Low

Antibodies in assay

HPA009420, HPA012769
 

Antibody HPA009420

 

Antibody HPA012769

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoskeleton (Intermediate filaments) 

Staining characteristics

Aggregate  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoskeleton (intermediate filaments).
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of cytoskeleton (intermediate filaments) in A-431. Weak granular staining of cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Nucleus but not nucleoli