MUC16

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA050015

 

Antibody CAB055172

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 DakoCytomation
 

Product name

HPA050015 M3520 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Supernatant 

Released in version

10 10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Native protein 

Length (aa)

23  

Antigen sequence

RTPDTSTMHLATSRTPASLSGPM
 
 

Matching transcripts

MUC16-001 - ENSP00000381008 [100%]
MUC16-002 - ENSP00000470885 [100%]
MUC16-003 - ENSP00000472883 [100%]
MUC16-004 - ENSP00000472781 [100%]
MUC16-201 - ENSP00000370338 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in actin filaments.
More information
 Application not done for this antibody. 

Antibody dilution

1:50  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

1519.2, 148.2, 136.0, 127.9, 74.1 1519.2, 148.2, 136.0, 127.9, 74.1 

Antibody dilution

1:250 1:500 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:2000  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human uterus, pre-menopause shows strong cytoplasmic and membranous positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:200 

Literature conformity

 Consistent with extensive gene/protein characterization data 

RNA consistency

 Consistent with RNAseq data 
 

ANTIGEN VIEW

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MUC16-001
 
MUC16-002
 
MUC16-003
 
MUC16-004
 
MUC16-201