Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Nuclear membrane

Additional location(s)

 

Staining summary

Localized to the nucleus or nuclear membrane.

Reliability (APE)

Low

Antibodies in assay

HPA040088, HPA040511
 

Antibody HPA040088

 

Antibody HPA040511

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nuclear membrane 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nuclear membrane 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nuclear membrane 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nuclear membrane.
 

Summary

Staining of nucleus in all three cell lines.   Staining of nuclear membrane in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nuclear membrane
Nucleus but not nucleoli