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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Cytoskeleton (Actin filaments) 

Staining summary

Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of cytoskeleton (actin filaments) in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA025241
 

Antibody HPA025241

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of cytoskeleton (actin filaments) in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane