Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Centrosome 

Staining summary

Localized to the cytoplasm and centrosome.

Reliability (APE)

Low

Antibodies in assay

CAB002496, HPA013316
 

Antibody CAB002496

 

Antibody HPA013316

 

         Cell line A-431

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Location(s)

 Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Weak 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm, Centrosome 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Cytoplasm, Centrosome 

Staining characteristics

 Granular 

Staining intensity

Negative Weak 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm & centrosome.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines withh additional staining of centrosome in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm