Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA003505, HPA003881, CAB018768
 

Antibody HPA003505

 

Antibody HPA003881

 

Antibody CAB018768

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli   Nucleus but not nucleoli 

Staining characteristics

Granular    

Staining intensity

Strong   Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

 Granular  

Staining intensity

Moderate Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of the nucleus in all three cell lines.   Staining of the nucleus in U-251 MG and U-2 OS.   Staining of the nucleus in all cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli