Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus

Additional location(s)

Golgi apparatus 

Staining summary

Staining of nucleus (and bright spots within) in all three cell lines. Additional staining of the Golgi apparatus in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA000843
 

Antibody HPA000843

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus 

Staining characteristics

Smooth 

Staining intensity

Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Nucleus 

Staining characteristics

Spotty 

Staining intensity

Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

Granular, Spotty 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
 

Summary

Staining of nucleus (and bright spots within) in all three cell lines. Additional staining of the Golgi apparatus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Golgi apparatus
Nucleus