Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA002190, CAB010454, HPA024386
 

Antibody HPA002190

 

Antibody CAB010454

 

Antibody HPA024386

 

         Cell line A-431

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Location(s)

Nucleus Nucleus but not nucleoli  

Staining characteristics

Spotty   

Staining intensity

Moderate Weak Negative 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

    

Staining characteristics

    

Staining intensity

  Negative Negative 

Validation

  
No staining.
 
No staining.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus Nucleus but not nucleoli  

Staining characteristics

Spotty   

Staining intensity

Weak Weak Negative 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Summary

Staining of spots in nuclei in U-251MG and A-431.   Staining of nuclei but not nucleoli in U-251 MG and A-431.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus