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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Golgi apparatus, Centrosome 

Staining summary

Mainly localized to the nucleus. In addition localized to centrosome and the Golgi apparatus. Caution: Based on antibodies targeting proteins from multiple genes.

Reliability (APE)

Low

Antibodies in assay

HPA041264, HPA042822, HPA043260
 

Antibody HPA041264

 

Antibody HPA042822

 

Antibody HPA043260

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Centrosome   

Staining characteristics

    

Staining intensity

Negative Weak   

Validation

The multi-targeting antibody not yielding a staining pattern.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
   

Comment

    

         Cell line MCF-7

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Location(s)

    Nucleus but not nucleoli 

Staining characteristics

    Spotty 

Staining intensity

    Moderate 

Validation

    
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

     

         Cell line U-2 OS

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Location(s)

   Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

   Spotty 

Staining intensity

Negative   Moderate 

Validation

The multi-targeting antibody not yielding a staining pattern.
   
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Centrosome Nucleus but not nucleoli, Golgi apparatus 

Staining characteristics

  Spotty 

Staining intensity

Negative Weak Weak 

Validation

The multi-targeting antibody not yielding a staining pattern.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in centrosome.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & golgi apparatus.
 

Summary

No staining was observed in any of the three cell lines.   Staining of centrosome in A-431 and U-251 MG with additional staining of nucleus in A-431.   Staining of nucleus in all three cell lines with additional staining of the Golgi apparatus in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Golgi apparatus
Nucleus but not nucleoli