Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli 

Staining summary

Localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

CAB002224, HPA008736, CAB025546
 

Antibody CAB002224

 

Antibody HPA008736

 

Antibody CAB025546

 

         Cell line A-431

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Location(s)

Nucleoli Nucleus but not nucleoli  

Staining characteristics

Cluster Spotty  

Staining intensity

Moderate Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleoli Nucleus  

Staining characteristics

Cluster Spotty  

Staining intensity

Weak Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli Nucleus but not nucleoli  

Staining characteristics

Spotty Spotty  

Staining intensity

Strong Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Staining of nucleoli in all three cell lines.   Staining of nucleus in all three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleoli
Nucleus