Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

Low

Antibodies in assay

CAB004288, HPA021676
 

Antibody CAB004288

 

Antibody HPA021676

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

   

Staining characteristics

   

Staining intensity

Negative   

Validation

No staining.
   

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus in A-431 and nucleus and cytoplasm in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli