Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli, Plasma membrane, Golgi apparatus

Additional location(s)

 

Staining summary

Localized to the nucleus and plasma membrane or to nucleoli and the Golgi apparatus.

Reliability (APE)

Very low

Antibodies in assay

HPA003254, CAB019381
 

Antibody HPA003254

 

Antibody CAB019381

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane Golgi apparatus 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane Nucleoli, Golgi apparatus 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane Nucleoli, Golgi apparatus 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & golgi apparatus.
 

Summary

Staining of nucleus and plasma membrane in all three cell lines.   Staining of cytoplasm and nucleus in U-251 MG and U-2 OS and of Golgi apparatus in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Golgi apparatus
Nucleoli
Nucleus but not nucleoli
Plasma membrane