Brain tissue expression of RPL27A in pons

PONS - Expression summary

Protein expressionⁱ

No data

RNA expressionⁱ

HPA Brain:	289.3	nTPM
FANTOM5:	50.6	Scaled Tags Per Million

Macaque:	59.6	nTPM
Mouse:	62.7	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	289.3

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 271.0

2188	Female, age 91, both hemispheres	317.0
2090	Male, age 80, both hemispheres	301.9
2301	Female, age 85, both hemispheres	248.6
1984	Male, age 76, both hemispheres	216.4

Dorsal cochlear nucleus
nTPM: 260.0

2802	Male, age 74, right hemisphere	303.2
2187	Female, age 91, both hemispheres	287.5
2378	Male, age 80, both hemispheres	251.6
2370	Male, age 61, both hemispheres	249.0
2058	Male, age 76, both hemispheres	208.7

Dorsal tegmental nucleus
nTPM: 287.8

2358	Male, age 61, both hemispheres	345.0
2083	Male, age 80, both hemispheres	304.3
2326	Male, age 74, both hemispheres	279.5
1936	Male, age 76, left hemisphere	222.3

Dorsolateral tegmental area
nTPM: 280.3

2337	Male, age 61, both hemispheres	385.3
2654	Male, age 80, both hemispheres	321.5
2751	Female, age 85, both hemispheres	255.4
2360	Male, age 74, both hemispheres	231.2
2141	Male, age 76, both hemispheres	208.1

Kolliker-Fuse nucleus
nTPM: 282.5

2315	Male, age 76, right hemisphere	314.7
2793	Female, age 91, left hemisphere	286.9
2163	Male, age 80, both hemispheres	246.0

Lateral lemniscal nuclei
nTPM: 203.5

2169	Male, age 76, both hemispheres	225.3
2746	Female, age 85, left hemisphere	212.3
2320	Female, age 91, left hemisphere	205.6
2036	Male, age 80, both hemispheres	192.3
2453	Male, age 74, right hemisphere	182.3

Lateral parabrachial nucleus
nTPM: 272.6

2792	Male, age 76, both hemispheres	317.0
2013	Male, age 80, both hemispheres	302.7
2139	Female, age 85, right hemisphere	244.5
2790	Male, age 61, both hemispheres	226.1

Lateral vestibular nucleus
nTPM: 252.8

2311	Male, age 80, both hemispheres	304.4
2184	Male, age 76, both hemispheres	201.2

Locus coeruleus
nTPM: 233.7

2720	Male, age 74, both hemispheres	285.0
2788	Male, age 74, unknown	248.8
2162	Male, age 80, both hemispheres	241.1
2123	Female, age 91, both hemispheres	239.8
2317	Female, age 72, unknown	218.3
2014	Male, age 55, unknown	206.6
1980	Male, age 76, both hemispheres	196.0

Medial parabrachial nucleus
nTPM: 261.5

2170	Female, age 91, both hemispheres	331.5
2854	Male, age 80, both hemispheres	278.8
2786	Male, age 76, both hemispheres	241.5
2156	Male, age 61, both hemispheres	232.0
2463	Female, age 85, right hemisphere	223.8

Medial periolivary nuclei
nTPM: 215.7

2081	Male, age 80, both hemispheres	240.5
2063	Male, age 76, both hemispheres	236.3
2458	Female, age 91, both hemispheres	226.2
2791	Male, age 74, right hemisphere	213.4
2171	Female, age 85, both hemispheres	162.0

Motor facial nucleus
nTPM: 217.5

2795	Female, age 91, both hemispheres	253.9
2697	Female, age 85, both hemispheres	235.3
2365	Male, age 74, right hemisphere	227.4
2126	Male, age 61, both hemispheres	226.7
2062	Male, age 76, both hemispheres	197.0
2327	Male, age 80, both hemispheres	164.9

Motor trigeminal nucleus
nTPM: 229.8

2335	Male, age 61, both hemispheres	275.7
2309	Male, age 76, both hemispheres	237.9
2321	Female, age 91, both hemispheres	229.0
2560	Male, age 74, both hemispheres	219.4
2079	Male, age 80, both hemispheres	186.9

Nuclei of the trapezoid body
nTPM: 200.3

1983	Male, age 76, both hemispheres	265.2
2166	Male, age 80, both hemispheres	205.2
2159	Male, age 61, both hemispheres	192.1
2368	Female, age 91, both hemispheres	176.3
2735	Female, age 85, both hemispheres	162.7

Nucleus raphe magnus
nTPM: 199.7

2051	Male, age 76, both hemispheres	263.3
2455	Male, age 74, both hemispheres	202.9
2328	Male, age 80, both hemispheres	194.8
2459	Female, age 91, both hemispheres	193.2
2160	Male, age 61, both hemispheres	177.8
2172	Female, age 85, both hemispheres	166.0

Paramedian reticular nucleus
nTPM: 265.9

2052	Male, age 76, both hemispheres	365.6
2057	Male, age 80, both hemispheres	238.6
2050	Female, age 85, both hemispheres	232.1
2313	Male, age 61, both hemispheres	227.4

Pedunculopontine tegmental nucleus
nTPM: 289.3

2338	Male, age 61, both hemispheres	386.1
2334	Male, age 80, both hemispheres	267.4
1921	Female, age 85, both hemispheres	214.5

Pontine nuclei
nTPM: 220.8

2319	Female, age 91, both hemispheres	263.3
1863	Male, age 55, unknown	245.0
2115	Male, age 80, left hemisphere	239.4
2109	Female, age 72, unknown	215.8
2787	Female, age 85, both hemispheres	213.2
1968	Male, age 76, left hemisphere	210.2
2454	Male, age 74, left hemisphere	197.6
2727	Male, age 61, right hemisphere	182.1

Pontine raphe nucleus
nTPM: 223.2

2853	Male, age 76, midline	239.7
2122	Female, age 85, midline	206.8

Posteroventral cochlear nucleus
nTPM: 288.0

2341	Male, age 80, both hemispheres	368.4
2135	Female, age 91, both hemispheres	345.0
2371	Male, age 61, both hemispheres	260.3
2070	Male, age 76, both hemispheres	246.9
2752	Female, age 85, both hemispheres	219.1

Principal sensory trigeminal nucleus
nTPM: 199.5

2367	Female, age 91, left hemisphere	236.5
2324	Male, age 76, both hemispheres	203.7
2158	Male, age 61, both hemispheres	192.8
2008	Male, age 80, left hemisphere	165.2

Reticular pontine nucleus, caudal
nTPM: 250.3

2457	Female, age 91, both hemispheres	288.2
2559	Male, age 74, both hemispheres	276.1
2092	Male, age 76, both hemispheres	270.1
2332	Male, age 80, right hemisphere	166.9

Reticular pontine nucleus, oral
nTPM: 264.2

2361	Male, age 74, both hemispheres	283.5
1939	Male, age 80, both hemispheres	279.8
2366	Female, age 91, both hemispheres	276.9
2167	Male, age 76, both hemispheres	273.1
2012	Female, age 85, both hemispheres	207.8

Reticulotegmental nucleus
nTPM: 225.9

2452	Male, age 74, both hemispheres	261.7
2322	Female, age 91, both hemispheres	231.5
2333	Male, age 80, both hemispheres	217.7
2325	Male, age 76, both hemispheres	192.8

Spinal trigeminal nucleus, oral
nTPM: 225.1

2164	Male, age 80, both hemispheres	248.6
1978	Male, age 76, both hemispheres	239.8
2737	Female, age 85, both hemispheres	186.9

Subcoeruleus area
nTPM: 240.6

2451	Male, age 74, both hemispheres	286.0
2369	Female, age 85, left hemisphere	275.2
1958	Male, age 80, both hemispheres	241.9
2323	Female, age 91, left hemisphere	212.3
2089	Male, age 76, left hemisphere	187.6

Superior olive
nTPM: 210.7

2020	Male, age 76, both hemispheres	250.0
2561	Female, age 91, both hemispheres	221.9
2364	Male, age 74, right hemisphere	208.5
1875	Female, age 85, both hemispheres	189.8
2329	Male, age 80, right hemisphere	183.4

Ventral periolivary nuclei
nTPM: 218.9

2023	Male, age 76, both hemispheres	273.7
2794	Female, age 91, left hemisphere	223.0
2165	Male, age 80, both hemispheres	208.7
2130	Female, age 85, both hemispheres	170.4

Ventrolateral tegmental area, A5 NE cell group
nTPM: 231.6

2359	Male, age 61, both hemispheres	283.7
2161	Male, age 80, both hemispheres	247.0
2840	Female, age 85, both hemispheres	243.4
1959	Male, age 76, both hemispheres	237.5
2456	Female, age 91, both hemispheres	192.7
2449	Male, age 74, both hemispheres	185.6

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	50.6

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 50.6

FF:10182-103D2	60 years, female	53.8
FF:10165-103B3	76 years, female	47.4

Pons
Scaled Tags Per Million: 26.4

FF:10033-101E6

18-54 years, mixed

26.4

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	59.6

Macaque sample

Sample description

nTPM

Pons
nTPM: 59.6

Samples: 2

Max nTPM: 68.9
Min nTPM: 50.3

Sample A		50.3
Sample B		68.9

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	62.7

Mouse sample

Sample description

nTPM

Pons
nTPM: 62.7

Samples: 4

Max nTPM: 64.8
Min nTPM: 58.9

Sample A		64.0
Sample B		58.9
Sample C		64.8
Sample D		63.0