Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cell Junctions

Additional location(s)

Cytoplasm, Golgi apparatus 

Staining summary

Mainly localized to the cell junctions. In addition localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA001636, HPA001637, CAB010822
 

Antibody HPA001636

 

Antibody HPA001637

 

Antibody CAB010822

 

         Cell line A-431

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Location(s)

Cytoplasm, Cell Junctions Cytoplasm, Cell Junctions Cell Junctions 

Staining characteristics

   

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm, Cell Junctions Cytoplasm, Cell Junctions Cell Junctions 

Staining characteristics

   

Staining intensity

Moderate Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm, Cell Junctions Cytoplasm, Cell Junctions Cell Junctions 

Staining characteristics

   

Staining intensity

Moderate Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cell junctions.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cell junctions.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cell junctions.
 

Summary

Staining of cytoplasm and cell junctions in all three cell lines with additional staining of plasma membrane in U-251 MG.   Staining of cytoplasm and cell junctions in all three cell lines.   Staining of cell junctions in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cell junctions
Cytoplasm
Golgi apparatus