Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Plasma membrane 

Staining summary

Mainly localized to cytoplasm but also nucleoli and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

HPA035022, HPA035023
 

Antibody HPA035022

 

Antibody HPA035023

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm   

Staining characteristics

   

Staining intensity

Moderate   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular, Spotty  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleoli in U-251 MG and A-431.   Staining of cytoplasm in U-251 MG. Staining of cytoplasm and plasma membrane in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleoli
Plasma membrane