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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus but excluded from the nucleoli in all three cell lines.

Reliability (APE)

High

Antibodies in assay

HPA027406, HPA027417, HPA027452
 

Antibody HPA027406

 

Antibody HPA027417

 

Antibody HPA027452

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Moderate Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Strong Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines.   Staining of nuclei but not nucleoli in all three cell lines.   Staining of the nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli