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SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Golgi apparatus 

Staining summary

Mainly localized to the nuclear membrane and cytoplasm. In addition localized to the Golgi apparatus and nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA026078, CAB026382
 

Antibody HPA026078

 

Antibody CAB026382

 

         Cell line A-431

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Location(s)

Nuclear membrane, Cytoplasm, Golgi apparatus  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm, Golgi apparatus  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Nuclear membrane, Cytoplasm  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nuclear membrane, cytoplasm & golgi apparatus.
 

Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Summary

Staining of nuclear membrane and cytoplasm in all three cell lines with additional staining of the Golgi apparatus in A-431 and U-2 OS.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nuclear membrane
Nucleus but not nucleoli