Fields »

SUBCELLULAR LOCATION

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Main location(s)

Vesicles

Additional location(s)

Golgi apparatus 

Staining summary

Mainly localized to vesicles. Caution: Based on antibodies targeting proteins from multiple genes.

Reliability (APE)

High

Antibodies in assay

HPA041019, HPA042033
 

Antibody HPA041019

 

Antibody HPA042033

 

         Cell line A-431

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Location(s)

Vesicles Vesicles 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Vesicles Vesicles 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleoli, Vesicles Vesicles 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles.
 

Summary

Staining of vesicles in all three cell lines with additional staining of nucleoli in U-251 MG.   Staining of vesicles in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Golgi apparatus
Endosome