Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

Medium

Antibodies in assay

CAB002302, HPA014539, HPA015310
 

Antibody CAB002302

 

Antibody HPA014539

 

Antibody HPA015310

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli Nucleus 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli Nucleus 

Staining characteristics

   

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli  

Staining characteristics

   

Staining intensity

Negative Weak Negative 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nuclei but not nucleoli in all three cell lines.   Staining of nuclei in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus