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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane

Additional location(s)

Cytoskeleton (Actin filaments), Focal Adhesions 

Staining summary

Staining of nucleus and plasma membrane in all three cell lines with additional staining of focal adhesions in U-251 MG and cytoskeleton (actin filaments) in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA038546
 

Antibody HPA038546

 

         Cell line A-431

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Location(s)

Nucleus, Plasma membrane, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Focal Adhesions 

Staining characteristics

Speckle 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, plasma membrane & cytoskeleton (actin filaments).
 

Summary

Staining of nucleus and plasma membrane in all three cell lines with additional staining of focal adhesions in U-251 MG and cytoskeleton (actin filaments) in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Actin filaments
Focal adhesions
Nucleus
Plasma membrane