Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Centrosome

Additional location(s)

Nucleoli, Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of nucleus in all three cell lines. Additional staining of centrosome in A-431 and U-251 MG. Additional staining of cytoskeleton (intermediate filaments) in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA028112
 

Antibody HPA028112

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Centrosome 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli, Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments), Centrosome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus, nucleoli & centrosome.
 

Summary

Staining of nucleus in all three cell lines. Additional staining of centrosome in A-431 and U-251 MG. Additional staining of cytoskeleton (intermediate filaments) in U-251 MG and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Intermediate filaments
Nucleoli
Nucleus