Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the plasma membrane. In addition localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

CAB000035, HPA018530
 

Antibody CAB000035

 

Antibody HPA018530

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Strong 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane.
 

Summary

Staining of plasma membrane in A-431 and U-251 MG.   Staining of plasma membrane in A-431 and U-251 MG. Staining of cytoplasm in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane