Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Cytoplasm, Mitochondria

Additional location(s)

 

Staining summary

Localized to the nucleus, cytoplasm and mitochondria.

Reliability (APE)

Very low

Antibodies in assay

HPA002119, HPA005779
 

Antibody HPA002119

 

Antibody HPA005779

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Cytoplasm, Mitochondria Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Cytoplasm, Mitochondria Nucleus but not nucleoli 

Staining characteristics

 Granular 

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of mitochondria in U-251 MG and U-2 OS.   Staining of nucleus in U-251MG, cytoplasm in U-2 OS and both nucleus and cytoplasm in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli