Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Plasma membrane, Cell Junctions 

Staining summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in U-2 OS and plasma membrane and cell junctions in A-431.

Reliability (Single)

Antibodies in assay

HPA019339
 

Antibody HPA019339

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cell Junctions 

Staining characteristics

Speckle 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Speckle 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Speckle 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane, cytoplasm & cell junctions.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of plasma membrane in U-2 OS and plasma membrane and cell junctions in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cell junctions
Cytoplasm
Nucleus but not nucleoli
Plasma membrane