Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus 

Staining summary

Localized to cytoplasm and nucleus.

Reliability (APE)

Medium

Antibodies in assay

HPA003387, CAB003799
 

Antibody HPA003387

 

Antibody CAB003799

 

         Cell line A-431

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Location(s)

Nucleus, Cytoplasm  

Staining characteristics

Smooth  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm   

Staining characteristics

Smooth   

Staining intensity

Strong   

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus, Cytoplasm  

Staining characteristics

Smooth  

Staining intensity

Moderate Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Staining of cytoplasm and nucleus in a fraction of the cells in all three cell lines.   No staining was observed in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus