Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to nuclear speckles.

Reliability (APE)

High

Antibodies in assay

HPA030902, HPA030903
 

Antibody HPA030902

 

Antibody HPA030903

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Speckle Speckle 

Staining intensity

Strong Moderate 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Speckle Speckle 

Staining intensity

Strong Strong 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Vesicles 

Staining characteristics

Speckle Speckle 

Staining intensity

Strong Strong 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines.   Staining of nucleus in all three cell lines with additional staining of vesicles in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli