Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

Localized to the nucleus but excluded from the nucleoli. Caution: Based on antibodies targeting proteins from multiple genes.

Reliability (APE)

Low

Antibodies in assay

CAB003695, HPA036174, HPA036175
 

Antibody CAB003695

 

Antibody HPA036174

 

Antibody HPA036175

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  Speckle Spotty 

Staining intensity

  Moderate Weak 

Validation

  
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 

Comment

    

         Cell line U-2 OS

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Location(s)

  Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  Speckle Spotty 

Staining intensity

  Weak Moderate 

Validation

  
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 

Comment

    

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

 Speckle Speckle 

Staining intensity

Negative Weak Weak 

Validation

No staining.
 
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern not consistent with available gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Nucleus but not nucleoli