The human protein atlas blog


Validation of antibodies using GFP-tagged target

2015-12-15
Cell Atlas Co-localization HPA14 Immunocytochemistry Immunofluorescence Subcell Atlas Validation

Antibody HPA37362 targeting BOD1L1 overlaps with GFP-tagged target protein and the localization is identical to that of endogenous protein in non-transfected control cells

Version 14 of The Human Protein Atlas includes a new type of validation of antibodies that are used for determining the subcellular localization of a protein.

A set of antibodies have been analyzed in transgenic cell lines expressing GFP-tagged target protein at near-endogenous levels to confirm that the antibodies are capable of binding the target protein. The approved antibodies are then used to determine the subcellular localization of endogenous protein in a selection of cell lines. A high validation score is assigned to those genes where the same location(s) are observed for both tagged protein and protein detected using labelled antibody in non-transfected cells...Read more


New investment in Life Science research

2015-12-11
AstraZeneca Life Science Wallenberg WCPR

Great news for Life Science researchers in Sweden and the new Wallenberg Center for Protein Research (WCPR).

AstraZeneca and the Knut and Alice Wallenberg Foundation support a new center for protein research, the Wallenberg Center for Protein Research (WCPR). Three of Swedens top Universities, KTH - Royal Institute of Technology, Uppsala University, and Chalmers Technical Univeristy, will work together to improve human health by developing new biological molecules, antibodies and peptides...Read more


Solid-phase cloning for high-throughput assembly

2015-12-08
DNA Expression vector High-throughput cloning

Attachment of DNA fragments onto a growing strand of DNA linked to a paramagnetic bead

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts

Simple and automated workflows for DNA assembly are necessary in large-scale projects such as the Human Protein Atlas. For this reason, we have developed two automated methods for swift and convenient high-throughput assembly of DNA into expression vector constructs.

By using paramagnetic beads as a support for the DNA, we can perform construct assembly on a liquid handler robot and furthermore eliminate the need for prior purification of PCR products by spin or gel purification...Read more


A prognosis based classification of uterin cancer

2015-12-01
Cancer Microarray Tissue Atlas Undifferentiated uterine sarcoma

The ovaries, fallopian tubes, and uterus

A prognosis based classification of undifferentiated uterine sarcomas: Identification of mitotic index, hormone receptors and YWHAE-FAM22 translocation status as predictors of survival

Undifferentiated uterine sarcomas (UUS) are rare tumors with a heterologous biology and a poor prognosis.

In a recent study by scientists from the Human Protein Atlas project and colleagues, the relevance of clinicopathology, mitotic index, translocation status (YWHAE-FAM22), and a number of biomarker candidates were examined for correlation with the prognosis of these tumors. The protein biomarkers P53, P16, Ki-67, Cyclin-D1, ER, PR, and ANLN were evaluated by immunohistochemistry...Read more


Human Protein Atlas talks and posters in December

2015-11-30
Poster Presentation

  • December 1-2

    Human Quantitative Dynamics

    Washington DC, USA

    Presenter: Mathias UhlÚn

    Talk: The Human Protein Atlas

    Human Quantitative Dynamics

  • December 3-6

    The 7th Annual Meeting of Proteomic Society, India - 2015

    Vellore, India

    Presenter: Prof. Peter Nilsson, KTH Royal Institute of Technology, Sweden

    Talk: Human Protein Atlas enabled neuroproteomic profiling of body fluids

    The 7th Annual Meeting of Proteomic Society

  • December 10, 4:40 PM
  • VI SCIENTIFIC MEETING ABOUT CLINICAL PROTEOMICS

    Salamanca, Spain

    Dr. Jochen M...Read more