Brain tissue expression of SNAP25 in pons

PONS - Expression summary

Mouse brain, Intensity
Ponsⁱ

19.845

RNA expressionⁱ

HPA Brain:	1336.9	nTPM
FANTOM5:	3585.3	Scaled Tags Per Million

Macaque:	1728.4	nTPM
Mouse:	973.6	nTPM
Rat:	1242.7	nTPM
Pig:	756.1	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - Mouse brain profileⁱ Protein expression in mouse brain using immunofluorescence is reported as fluorescence intensity. , antibody HPA001830
Pons mean intensity	19.845
	intensityⁱ Protein expression in mouse brain using immunofluorescence is reported as mean fluorescence intensity.	FRONT BACK
Barrington's nucleus	-
Koelliker-fuse nucleus	-
Motor tregiminal nucleus	7.281
Parabrachial nucleus	19.845
Principle sensory trigeminal nucleus	12.420
Locus coeruleus	-
Pontine nucleus	-
Vestibular nucleus	4.069
Pontine reticular nucleus, oral	-
Lateral lemniscus	9.654
Superior paraolivary nucleus	5.246

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1336.9

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 495.5

1984	Male, age 76, both hemispheres	707.6
2301	Female, age 85, both hemispheres	543.8
2188	Female, age 91, both hemispheres	461.6
2090	Male, age 80, both hemispheres	268.8

Dorsal cochlear nucleus
nTPM: 324.6

2058	Male, age 76, both hemispheres	403.7
2370	Male, age 61, both hemispheres	373.8
2187	Female, age 91, both hemispheres	368.0
2378	Male, age 80, both hemispheres	264.1
2802	Male, age 74, right hemisphere	213.2

Dorsal tegmental nucleus
nTPM: 368.3

2083	Male, age 80, both hemispheres	432.7
1936	Male, age 76, left hemisphere	407.3
2358	Male, age 61, both hemispheres	390.5
2326	Male, age 74, both hemispheres	242.8

Dorsolateral tegmental area
nTPM: 298.7

2654	Male, age 80, both hemispheres	409.2
2751	Female, age 85, both hemispheres	357.1
2141	Male, age 76, both hemispheres	327.5
2360	Male, age 74, both hemispheres	284.5
2337	Male, age 61, both hemispheres	115.3

Kolliker-Fuse nucleus
nTPM: 478.5

2793	Female, age 91, left hemisphere	565.9
2163	Male, age 80, both hemispheres	537.6
2315	Male, age 76, right hemisphere	332.0

Lateral lemniscal nuclei
nTPM: 956.6

2169	Male, age 76, both hemispheres	1250.8
2453	Male, age 74, right hemisphere	1154.4
2036	Male, age 80, both hemispheres	941.2
2746	Female, age 85, left hemisphere	806.6
2320	Female, age 91, left hemisphere	629.8

Lateral parabrachial nucleus
nTPM: 573.6

2139	Female, age 85, right hemisphere	1013.6
2790	Male, age 61, both hemispheres	542.6
2013	Male, age 80, both hemispheres	523.9
2792	Male, age 76, both hemispheres	214.3

Lateral vestibular nucleus
nTPM: 439.0

2184	Male, age 76, both hemispheres	539.0
2311	Male, age 80, both hemispheres	339.0

Locus coeruleus
nTPM: 529.0

2720	Male, age 74, both hemispheres	867.9
2123	Female, age 91, both hemispheres	823.9
2788	Male, age 74, unknown	433.0
2014	Male, age 55, unknown	426.0
2162	Male, age 80, both hemispheres	421.3
1980	Male, age 76, both hemispheres	378.0
2317	Female, age 72, unknown	352.9

Medial parabrachial nucleus
nTPM: 453.5

2854	Male, age 80, both hemispheres	671.5
2156	Male, age 61, both hemispheres	493.1
2463	Female, age 85, right hemisphere	383.2
2786	Male, age 76, both hemispheres	365.8
2170	Female, age 91, both hemispheres	353.9

Medial periolivary nuclei
nTPM: 885.4

2171	Female, age 85, both hemispheres	1478.7
2063	Male, age 76, both hemispheres	1267.8
2791	Male, age 74, right hemisphere	723.0
2458	Female, age 91, both hemispheres	520.1
2081	Male, age 80, both hemispheres	437.5

Motor facial nucleus
nTPM: 888.8

2062	Male, age 76, both hemispheres	1305.6
2365	Male, age 74, right hemisphere	1059.6
2327	Male, age 80, both hemispheres	991.0
2697	Female, age 85, both hemispheres	922.4
2126	Male, age 61, both hemispheres	554.9
2795	Female, age 91, both hemispheres	499.0

Motor trigeminal nucleus
nTPM: 545.0

2079	Male, age 80, both hemispheres	848.2
2560	Male, age 74, both hemispheres	509.0
2335	Male, age 61, both hemispheres	486.9
2321	Female, age 91, both hemispheres	461.9
2309	Male, age 76, both hemispheres	418.9

Nuclei of the trapezoid body
nTPM: 1243.6

2159	Male, age 61, both hemispheres	2248.3
2735	Female, age 85, both hemispheres	1397.3
1983	Male, age 76, both hemispheres	1192.5
2166	Male, age 80, both hemispheres	780.1
2368	Female, age 91, both hemispheres	599.7

Nucleus raphe magnus
nTPM: 1336.9

2160	Male, age 61, both hemispheres	2351.1
2455	Male, age 74, both hemispheres	1727.7
2172	Female, age 85, both hemispheres	1547.4
2328	Male, age 80, both hemispheres	907.8
2051	Male, age 76, both hemispheres	790.3
2459	Female, age 91, both hemispheres	697.3

Paramedian reticular nucleus
nTPM: 718.7

2050	Female, age 85, both hemispheres	1134.3
2313	Male, age 61, both hemispheres	746.0
2057	Male, age 80, both hemispheres	586.6
2052	Male, age 76, both hemispheres	408.0

Pedunculopontine tegmental nucleus
nTPM: 441.8

2338	Male, age 61, both hemispheres	512.5
1921	Female, age 85, both hemispheres	417.7
2334	Male, age 80, both hemispheres	395.3

Pontine nuclei
nTPM: 1179.5

2727	Male, age 61, right hemisphere	2208.2
1863	Male, age 55, unknown	1501.6
2787	Female, age 85, both hemispheres	1386.2
2109	Female, age 72, unknown	1264.9
1968	Male, age 76, left hemisphere	1081.4
2454	Male, age 74, left hemisphere	969.0
2115	Male, age 80, left hemisphere	566.8
2319	Female, age 91, both hemispheres	458.1

Pontine raphe nucleus
nTPM: 689.5

2122	Female, age 85, midline	996.3
2853	Male, age 76, midline	382.7

Posteroventral cochlear nucleus
nTPM: 389.7

2752	Female, age 85, both hemispheres	769.4
2070	Male, age 76, both hemispheres	516.1
2371	Male, age 61, both hemispheres	333.9
2341	Male, age 80, both hemispheres	295.6
2135	Female, age 91, both hemispheres	33.5

Principal sensory trigeminal nucleus
nTPM: 970.7

2158	Male, age 61, both hemispheres	1873.0
2008	Male, age 80, left hemisphere	1016.3
2367	Female, age 91, left hemisphere	535.0
2324	Male, age 76, both hemispheres	458.4

Reticular pontine nucleus, caudal
nTPM: 668.9

2332	Male, age 80, right hemisphere	1056.7
2092	Male, age 76, both hemispheres	816.1
2457	Female, age 91, both hemispheres	497.1
2559	Male, age 74, both hemispheres	305.6

Reticular pontine nucleus, oral
nTPM: 589.4

2361	Male, age 74, both hemispheres	949.7
2012	Female, age 85, both hemispheres	775.0
2366	Female, age 91, both hemispheres	487.9
2167	Male, age 76, both hemispheres	382.9
1939	Male, age 80, both hemispheres	351.4

Reticulotegmental nucleus
nTPM: 563.0

2325	Male, age 76, both hemispheres	717.5
2333	Male, age 80, both hemispheres	581.7
2322	Female, age 91, both hemispheres	528.1
2452	Male, age 74, both hemispheres	424.5

Spinal trigeminal nucleus, oral
nTPM: 529.9

2737	Female, age 85, both hemispheres	641.8
2164	Male, age 80, both hemispheres	499.3
1978	Male, age 76, both hemispheres	448.6

Subcoeruleus area
nTPM: 566.7

2323	Female, age 91, left hemisphere	758.6
2089	Male, age 76, left hemisphere	748.0
1958	Male, age 80, both hemispheres	734.1
2451	Male, age 74, both hemispheres	331.3
2369	Female, age 85, left hemisphere	261.6

Superior olive
nTPM: 866.8

1875	Female, age 85, both hemispheres	1256.6
2364	Male, age 74, right hemisphere	911.9
2329	Male, age 80, right hemisphere	775.2
2020	Male, age 76, both hemispheres	729.9
2561	Female, age 91, both hemispheres	660.4

Ventral periolivary nuclei
nTPM: 994.0

2130	Female, age 85, both hemispheres	1255.7
2165	Male, age 80, both hemispheres	1113.8
2794	Female, age 91, left hemisphere	900.1
2023	Male, age 76, both hemispheres	706.3

Ventrolateral tegmental area, A5 NE cell group
nTPM: 711.2

2449	Male, age 74, both hemispheres	1359.2
1959	Male, age 76, both hemispheres	823.5
2840	Female, age 85, both hemispheres	754.2
2161	Male, age 80, both hemispheres	681.6
2456	Female, age 91, both hemispheres	338.9
2359	Male, age 61, both hemispheres	309.6

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	3585.3

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 922.4

FF:10182-103D2	60 years, female	1229.2
FF:10165-103B3	76 years, female	615.5

Pons
Scaled Tags Per Million: 3585.3

FF:10033-101E6

18-54 years, mixed

3585.3

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1728.4

Macaque sample

Sample description

nTPM

Pons
nTPM: 1728.4

Samples: 2

Max nTPM: 1998.7
Min nTPM: 1458.1

Sample A		1998.7
Sample B		1458.1

PONS - Pig RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	756.1

Pig sample

Sample description

nTPM

Pons
nTPM: 756.1

Samples: 3

Max nTPM: 1067.2
Min nTPM: 499.4

Sample A	Female	1067.2
Sample B	Female	499.4
Sample C	Male	701.7

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	973.6

Mouse sample

Sample description

nTPM

Pons
nTPM: 973.6

Samples: 4

Max nTPM: 1080.8
Min nTPM: 888.4

Sample A		1080.8
Sample B		888.4
Sample C		1015.2
Sample D		910.1

PONS - Rat RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1242.7

Rat sample

Sample description

nTPM

Pons
nTPM: 1242.8

Samples: 4

Max nTPM: 1347.3
Min nTPM: 1120.4

Sample A		1239.4
Sample A		1347.3
Sample A		1120.4
Sample A		1263.9