Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Vesicles

Additional location(s)

 

Staining summary

Localized to the cytoplasm/plasma membrane or nucleus and vesicles.

Reliability (APE)

Very low

Antibodies in assay

HPA039105, HPA039828
 

Antibody HPA039105

 

Antibody HPA039828

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of cytoplasm and plasma membrane in all three cell lines.   Staining of nucleus and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane
Endosome