The human protein atlas blog
Solid-phase cloning for high-throughput assembly of single and multiple DNA parts
Simple and automated workflows for DNA assembly are necessary in large-scale projects such as the Human Protein Atlas. For this reason, we have developed two automated methods for swift and convenient high-throughput assembly of DNA into expression vector constructs.
By using paramagnetic beads as a support for the DNA, we can perform construct assembly on a liquid handler robot and furthermore eliminate the need for prior purification of PCR products by spin or gel purification...Read more