Brain tissue expression of GNAS in pons

PONS - Expression summary

Protein expressionⁱ

No data

RNA expressionⁱ

HPA Brain:	1969.4	nTPM
FANTOM5:	1935.0	Scaled Tags Per Million

Macaque:	1203.8	nTPM
Mouse:	1045.0	nTPM
Rat:	1477.2	nTPM
Pig:	1834.1	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1969.4

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 898.9

2301	Female, age 85, both hemispheres	995.8
2090	Male, age 80, both hemispheres	923.4
2188	Female, age 91, both hemispheres	840.2
1984	Male, age 76, both hemispheres	836.2

Dorsal cochlear nucleus
nTPM: 961.8

2058	Male, age 76, both hemispheres	1120.9
2187	Female, age 91, both hemispheres	1103.1
2370	Male, age 61, both hemispheres	1015.8
2802	Male, age 74, right hemisphere	845.0
2378	Male, age 80, both hemispheres	723.9

Dorsal tegmental nucleus
nTPM: 1119.3

2083	Male, age 80, both hemispheres	1341.2
2358	Male, age 61, both hemispheres	1197.7
1936	Male, age 76, left hemisphere	991.8
2326	Male, age 74, both hemispheres	946.6

Dorsolateral tegmental area
nTPM: 1107.6

2141	Male, age 76, both hemispheres	1334.8
2654	Male, age 80, both hemispheres	1295.4
2751	Female, age 85, both hemispheres	1115.8
2360	Male, age 74, both hemispheres	970.7
2337	Male, age 61, both hemispheres	821.3

Kolliker-Fuse nucleus
nTPM: 1003.3

2163	Male, age 80, both hemispheres	1321.1
2793	Female, age 91, left hemisphere	892.6
2315	Male, age 76, right hemisphere	796.2

Lateral lemniscal nuclei
nTPM: 1262.0

2036	Male, age 80, both hemispheres	1577.2
2169	Male, age 76, both hemispheres	1555.1
2746	Female, age 85, left hemisphere	1389.9
2453	Male, age 74, right hemisphere	1025.5
2320	Female, age 91, left hemisphere	762.4

Lateral parabrachial nucleus
nTPM: 1066.2

2139	Female, age 85, right hemisphere	1631.9
2013	Male, age 80, both hemispheres	1081.3
2790	Male, age 61, both hemispheres	1003.3
2792	Male, age 76, both hemispheres	548.4

Lateral vestibular nucleus
nTPM: 1098.1

2184	Male, age 76, both hemispheres	1134.7
2311	Male, age 80, both hemispheres	1061.6

Locus coeruleus
nTPM: 1969.4

2123	Female, age 91, both hemispheres	2870.6
2720	Male, age 74, both hemispheres	2110.6
2014	Male, age 55, unknown	2028.7
1980	Male, age 76, both hemispheres	1883.5
2317	Female, age 72, unknown	1785.5
2788	Male, age 74, unknown	1595.1
2162	Male, age 80, both hemispheres	1511.8

Medial parabrachial nucleus
nTPM: 1179.4

2854	Male, age 80, both hemispheres	1327.7
2170	Female, age 91, both hemispheres	1317.1
2156	Male, age 61, both hemispheres	1273.9
2786	Male, age 76, both hemispheres	1076.2
2463	Female, age 85, right hemisphere	902.3

Medial periolivary nuclei
nTPM: 1112.7

2171	Female, age 85, both hemispheres	1753.1
2063	Male, age 76, both hemispheres	1490.9
2791	Male, age 74, right hemisphere	894.8
2081	Male, age 80, both hemispheres	772.6
2458	Female, age 91, both hemispheres	652.2

Motor facial nucleus
nTPM: 1165.7

2062	Male, age 76, both hemispheres	1676.6
2697	Female, age 85, both hemispheres	1284.0
2365	Male, age 74, right hemisphere	1112.3
2327	Male, age 80, both hemispheres	1083.9
2126	Male, age 61, both hemispheres	1023.2
2795	Female, age 91, both hemispheres	814.5

Motor trigeminal nucleus
nTPM: 1077.0

2079	Male, age 80, both hemispheres	1278.0
2335	Male, age 61, both hemispheres	1227.3
2309	Male, age 76, both hemispheres	1136.7
2560	Male, age 74, both hemispheres	906.0
2321	Female, age 91, both hemispheres	836.9

Nuclei of the trapezoid body
nTPM: 1332.1

2735	Female, age 85, both hemispheres	1779.7
2159	Male, age 61, both hemispheres	1609.3
1983	Male, age 76, both hemispheres	1604.6
2166	Male, age 80, both hemispheres	1003.8
2368	Female, age 91, both hemispheres	663.1

Nucleus raphe magnus
nTPM: 1369.8

2172	Female, age 85, both hemispheres	1941.6
2160	Male, age 61, both hemispheres	1892.4
2455	Male, age 74, both hemispheres	1414.6
2051	Male, age 76, both hemispheres	1125.7
2328	Male, age 80, both hemispheres	1107.9
2459	Female, age 91, both hemispheres	736.9

Paramedian reticular nucleus
nTPM: 1470.7

2050	Female, age 85, both hemispheres	1668.6
2313	Male, age 61, both hemispheres	1665.1
2057	Male, age 80, both hemispheres	1558.7
2052	Male, age 76, both hemispheres	990.2

Pedunculopontine tegmental nucleus
nTPM: 1226.1

2334	Male, age 80, both hemispheres	1416.6
1921	Female, age 85, both hemispheres	1272.9
2338	Male, age 61, both hemispheres	988.6

Pontine nuclei
nTPM: 1402.8

2787	Female, age 85, both hemispheres	2461.5
1863	Male, age 55, unknown	1727.9
2727	Male, age 61, right hemisphere	1602.0
1968	Male, age 76, left hemisphere	1398.2
2109	Female, age 72, unknown	1384.5
2115	Male, age 80, left hemisphere	920.5
2319	Female, age 91, both hemispheres	881.5
2454	Male, age 74, left hemisphere	846.2

Pontine raphe nucleus
nTPM: 1551.6

2122	Female, age 85, midline	1762.2
2853	Male, age 76, midline	1341.1

Posteroventral cochlear nucleus
nTPM: 1015.4

2752	Female, age 85, both hemispheres	1534.7
2070	Male, age 76, both hemispheres	1245.4
2371	Male, age 61, both hemispheres	993.0
2341	Male, age 80, both hemispheres	693.9
2135	Female, age 91, both hemispheres	610.2

Principal sensory trigeminal nucleus
nTPM: 1213.4

2158	Male, age 61, both hemispheres	1641.0
2008	Male, age 80, left hemisphere	1377.5
2324	Male, age 76, both hemispheres	1039.0
2367	Female, age 91, left hemisphere	796.1

Reticular pontine nucleus, caudal
nTPM: 985.5

2092	Male, age 76, both hemispheres	1197.8
2332	Male, age 80, right hemisphere	1123.2
2559	Male, age 74, both hemispheres	934.1
2457	Female, age 91, both hemispheres	686.8

Reticular pontine nucleus, oral
nTPM: 1099.8

2012	Female, age 85, both hemispheres	1436.1
1939	Male, age 80, both hemispheres	1207.8
2167	Male, age 76, both hemispheres	1159.2
2361	Male, age 74, both hemispheres	955.5
2366	Female, age 91, both hemispheres	740.2

Reticulotegmental nucleus
nTPM: 1150.3

2325	Male, age 76, both hemispheres	1335.8
2452	Male, age 74, both hemispheres	1150.8
2333	Male, age 80, both hemispheres	1125.2
2322	Female, age 91, both hemispheres	989.2

Spinal trigeminal nucleus, oral
nTPM: 1155.6

2737	Female, age 85, both hemispheres	1262.7
1978	Male, age 76, both hemispheres	1168.4
2164	Male, age 80, both hemispheres	1035.7

Subcoeruleus area
nTPM: 1254.0

2323	Female, age 91, left hemisphere	1848.0
1958	Male, age 80, both hemispheres	1448.4
2089	Male, age 76, left hemisphere	1098.2
2451	Male, age 74, both hemispheres	1039.5
2369	Female, age 85, left hemisphere	835.6

Superior olive
nTPM: 1131.1

1875	Female, age 85, both hemispheres	1709.5
2020	Male, age 76, both hemispheres	1104.3
2364	Male, age 74, right hemisphere	1078.8
2329	Male, age 80, right hemisphere	984.3
2561	Female, age 91, both hemispheres	778.7

Ventral periolivary nuclei
nTPM: 1271.9

2130	Female, age 85, both hemispheres	1851.8
2165	Male, age 80, both hemispheres	1379.9
2023	Male, age 76, both hemispheres	1028.1
2794	Female, age 91, left hemisphere	827.6

Ventrolateral tegmental area, A5 NE cell group
nTPM: 1055.4

2840	Female, age 85, both hemispheres	1350.4
1959	Male, age 76, both hemispheres	1199.7
2449	Male, age 74, both hemispheres	1092.2
2359	Male, age 61, both hemispheres	1089.0
2161	Male, age 80, both hemispheres	983.9
2456	Female, age 91, both hemispheres	617.4

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	1935.0

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 1935.0

FF:10182-103D2	60 years, female	2227.5
FF:10165-103B3	76 years, female	1642.5

Pons
Scaled Tags Per Million: 1680.8

FF:10033-101E6

18-54 years, mixed

1680.8

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1203.8

Macaque sample

Sample description

nTPM

Pons
nTPM: 1203.8

Samples: 2

Max nTPM: 1291.6
Min nTPM: 1116.0

Sample A		1116.0
Sample B		1291.6

PONS - Pig RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1834.1

Pig sample

Sample description

nTPM

Pons
nTPM: 1834.1

Samples: 3

Max nTPM: 1927.9
Min nTPM: 1725.3

Sample A	Female	1849.1
Sample B	Female	1725.3
Sample C	Male	1927.9

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1045.0

Mouse sample

Sample description

nTPM

Pons
nTPM: 1045.0

Samples: 4

Max nTPM: 1136.2
Min nTPM: 992.7

Sample A		1057.1
Sample B		992.7
Sample C		1136.2
Sample D		993.9

PONS - Rat RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	1477.2

Rat sample

Sample description

nTPM

Pons
nTPM: 1477.2

Samples: 4

Max nTPM: 1579.2
Min nTPM: 1374.5

Sample A		1579.2
Sample A		1564.8
Sample A		1390.4
Sample A		1374.5