The human protein atlas blog


Systematic antibody validation using siRNA

2016-02-02
Cell Atlas Gene editing Immunocytochemistry Immunofluorescence siRNA Subcell Atlas

Fig 1a. The protein HNRNPUL1 is localized to the nucleus by staining with antibody HPA046290 (green)

Systematic antibody validation with siRNA for the Human Protein Atlas

Antibodies are among the most frequently used tools for basic research and clinical assays. For antibodies used in therapy or diagnostics, there are well-defined and strict guidelines that must be complied with before approval for clinical assays. For research antibodies, such guidelines have not yet been developed, despite the importance of demonstrating that they are specific, selective, and yield reproducible results in the immunoassay for which they are to be used...Read more